August 2012 - Made available in PDF format versions of Chapter 5: Salmonella and Appendix 1 (archived) from 2009 which were incorporated by reference in 21 CFR Parts 16 and 118: Federal Register Final Rule (July 9, 2009, 74 FR 33030): Prevention of Salmonella Enteritidis in Shell Eggs During Production, Storage, and Transportation. February 2014 - Section on Detection and isolation of Salmonella from shell eggs was replaced, and validation data and additional references were added in an Appendix.May 2014 - The VITEK 2 method of Presumptive generic identification of Salmonella was updated.December 2015 - A section for the Statens Serum Institute Procedure was added to Section E: Identification of Salmonella.Forstner, Laboratory Services, Minnesota Department of Agriculture). August 2016 - Added the Salmonella Flipbook, a pictorial general guide to aid analysts in the detection and identification of Salmonella growing on the plating media and screening tubes used in the BAM Chapter 5 Salmonella method.March 2018 - Added real-time quantitative PCR for confirmation of Salmonella isolates protocol and validated preenrichment changes for leafy produce, herbs and sprouts Vegetables removed from revised Section C7 Validated Preenrichment broth change in section C23.July 2018 - Section C7 revised to include vegetables not included in C23 or C27.November 2019 - Section E9 updated to include additional options for identification of Salmonella.November 2019 - Section C.10.c Added detailed procedure to prepare sample composites for oregano, cinnamon, allspice and cloves.Limited the foods covered by Section C7 to those not listed elsewhere in Section C. Added LAMP confirmation of Salmonella isolates protocol. Added LAMP screening method for animal food. Added sample set up for non-powder forms of allspice, cinnamon, cloves and oregano. use biochemical confirmation and typos in section E.3.a, E.3.b, E.8 and Reference 2. December 2020 - Minor changes in sections D.10.February 2021 - Removed text from section E.9.b.May 2021 - Section C.28 updated to include an alternative positive control, a video demonstration, and software updates for LAMP screening for Salmonella in animal food protocol Section E.9.d updated to include a video demonstration and software updates for LAMP confirmation of Salmonella isolates protocol Section C.7.a updated to include mushrooms.June 2021 - Section A.27 updated to include ultraviolet light source Section C.7 updated by changing nut/nut meat preparation procedure from blending to soaking Section C.23 updated by removing erroneous requirement to adjust the pH of preenrichment before incubation Section C29 added method for spent sprout irrigation water from alfalfa, mung bean, and broccoli varieties Section D.7 updated by adding Green Fluorescent Protein control strains.September 2021-Section D.7 updated to include serological-formulas of ATCC control cultures 1234 Section E.9.c updated to remove SmartCycler instructions from the Real-time PCR confirmation test.October 2021-Section C.2.c updated to correct a typo Section E.11 serotyping submission information and shipping point of contact updated.March 2022 - Section C.28 updated to include dairy feed as a validated animal food matrix, AB 7500 Fast as an alternative LAMP assay platform, two additional reagent choices for LAMP, and removed duplicate testing recommendation for LAMP screening of Salmonella in animal food protocol Section E.9.d updated to include AB 7500 Fast as an alternative LAMP assay platform, two additional reagent choices for LAMP, and removed duplicate testing recommendation for LAMP confirmation of Salmonella isolates protocol Section E.11 was updated by linking SeqSero to Protocols.IO.November 2022 – Sections C.28 and E.9 updated to include screenshots for AB 7500 Fast setup and representative Genie instrument outputs, recommended GspSSD2.0 as the preferred LAMP master mix, and updated template preparation method to include a final centrifugation step. Andrews (ret.), Hua Wang, Andrew Jacobson (ret.), Beilei Ge, Guodong Zhang, and Thomas Hammack The most recent Edition of BAM Chapter 5: Salmonella is available below this notice.Īuthors: Wallace H. These 2 documents are also available as a combined file (PDF, 382 Kb). If you are looking for BAM Chapter 5: Salmonella (December 2007 Edition) that is incorporated by reference in 21 CFR Parts 16 and 118: Federal Register Final Rule (July 9, 2009, 74 FR 33030): Prevention of Salmonella Enteritidis in Shell Eggs During Production, Storage, and Transportation, please use these versions of the BAM Salmonella Chapter (PDF, 189 Kb) and Appendix 1: Rapid Methods for Detecting Foodborne Pathogens (PDF, 195 Kb).
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